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N-linked Glycan Characterization and Profiling: Combining the Power of Accurate Mass, Reference Glucose Units, and UNIFI Software for Confident Glycan Assignments

27 Mar 2014

This application note details a new workflow for a glycan assay, using FLR with mass confirmation, available in the latest version of the Biopharmaceutical Platform Solution with UNIFI. The practical use of this workflow for fluorescent labeled (2AB) N-linked released glycan characterization is illustrated using a biosimilar/innovator biotherapeutic comparability study.

Biopharmaceutical Platform Solution with UNIFI

Waters

The Waters Biopharmaceutical Platform Solution with UNIFI® integrates robust UPLC®/MS characterization technology with the UNIFI Scientific Information System for applications in bioseparations, intact protein mass analysis, peptide mapping and released glycan analysis.This platform unites all aspects of biotherapeutic analyses and workflows, enabling organizations to apply high resolution analytics across the development process continuum. The platform captures complex mass spectrometry and chromatography data, and enables laboratories to comprehensively and routinely acquire, process, and share LC and MS characterization information throughout a biotherapeutic’s lifecycle. Results can be shared from discovery to development to GxP quality control.

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N-linked Glycan Characterization and Profiling: Combining the Power of Accurate Mass, Reference Glucose Units, and UNIFI Software for Confident Glycan Assignments