Application Note: Method Development for the Analysis of Endogenous Steroids Using Convergence Chromatography with Mass Spectrometric Detection
28 May 2014

Steroid biosynthesis is a complex metabolic pathway utilizing simple precursors to synthesize multiple steroidal forms. Precise and accurate steroid analysis is critical for the development of steroid-based therapeutics. For mass spectrometric analysis of steroids, due to their structural similarity, chromatographic separation of the steroids is essential prior to analysis. This study focuses on the application of convergence chromatography (CC), utilizing liquid CO 2 as the primary mobile phase, for the rapid chromatographic analysis of endogenous steroids.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions


Already a member?

Log in for full access to the article




Remember me

Company