Application Note: High Definition Multiple Reaction Monitoring: The Application of Quadrupole Ion Mobility Time-of-Flight Mass Spectrometry for Targeted Proteomics Studies
22 October 2013

Targeted LC/MS-based methods have been applied to quantitative proteomics, facilitating highly accurate and multiplexed relative and absolute quantification studies. Both triple quadrupole and high-resolution mass spectrometers have been used, with the key benefits being sensitivity, acquisition speed, and linear dynamic range. However the specificity of SRM/MRM peptide quantification has been questioned. This study examines high definition MRM (HD-MRM) workflow using the SYNAPT G2-Si.