Application Note: Applications of a Highly Uniform UV Illumination Imaging System for Quantitative DNA and Protein Analysis
29 March 2011
This application note by UVP reports on the development and characterisation of a highly uniform UV transillumination system. Quantitative analysis of proteins and nucleic acids is limited by the extreme lack of illumination uniformity across the surface of typical UV light boxes. Here use of a high-density lighting system with a tuned phosphor coating, uniformity of <5% coefficient of variance (CV) across the full imaging surface has been achieved and applied to proteomic analysis.