Using SapphireAmp® Fast PCR Master Mix for Rapid Colony PCR Screening

SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix. SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be completed in 1 hour, and amplifications of up to 6 kb are possible.This hot start PCR master mix product is especially well-suited for E. coli-based colony PCR to screen inserts up to ~5 kb in length. Colony PCR with SapphireAmp Master Mix can be completed in about 1 hour.SapphireAmp reactions can be assembled by simply adding primers and DNA template to the hot start PCR master mix. Reactions can be analyzed directly without purification; PCR products can be loaded directly onto a gel for electrophoresis or digested with restriction enzymes in the SapphireAmp reaction buffer.Features: Hot start PCR master mix is optimized for fast PCR Colony PCR can be completed in half the time required for conventional Taq DNA polymerase Convenient- eliminates purification steps with no reduction in yield Restriction enzyme digestion of the PCR products can be performed directly in the PCR buffer Reactions can be directly loaded onto a gel without purification or the addition of other reagents Applications: Hot start PCR Colony PCR Fast PCR