Application Note: Ultrapure Water Free From Biologically Active Impurities Suitable for PCR
11 January 2011

In this application note by ELGA LabWater, the requirement for ultrapure water free from contaminants that can impede DNA amplification in PCR is addressed. The need for reagents and solutions free from nucleases (DNase, RNase) which could cause breakdown of oligonucleotides is widely recognized, however, the influence of other waterborne contaminants, such as bacteria, ions and organic compounds, is rarely considered. ELGA’s PURELAB Ultra Genetic uses a multi-step purification process to produce ultrapure grade water suitable for PCR including Ultraviolet (UV) radiation and ultrafiltration.