Application Note: Successful RNA Interference Experiments in Difficult-to-Transfect Cell Lines
20 November 2013

Successful gene silencing experiments that help clarify a biological pathway rely on good experimental planning and design. RNA interference (RNAi) silencing methods borrow an elegant endogenous biological pathway that can be applied to modulate gene expression. Significant and relevant results depend on a number of factors in the workflow. In this application note the optimization is discussed, in particular the delivery of functional siRNAs and accurate confirmation of gene knockdown remain paramount.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions


Already a member?

Log in for full access to the article




Remember me