Optimization of a Bulk Reagent Liquid Handler for Low Volume Dispensing

2 Oct 2012

This application note describes the performance of Thermo Scientific Multidrop Combi nL in low volume and the operating principle of the valves. Multidrop Combi nL provides consistent, high-throughput dispensing into 1536-, 384- and 96-well microplates covering a volume range 50 nl - 50 µl. In this application note liquids with different surface tension properties were tested by dispensing multiple volumes into 1536- and 384-well plates. Descriptions of the liquid calibration and the valve correction feature with the Thermo Scientific FILLit Software are also presented.

Thermo Scientific Multidrop Combi nL

Thermo Fisher Scientific

The Thermo Scientific Multidrop Combi nL – A low volume bulk reagent dispenser, expanding the range of the market-leading Multidrop product family. The Multidrop Combi nL provides accurate and consistent high throughput reagent dispensing across a volume range of 50nL to 50µL. It offers low volume dispensing into 96-, 384- and 1536- well plates with excellent precision and accuracy. Fast dispensing combined with full robotic compatibility ensures increased assay throughput for laboratories needing low volume assay formats. Key Features: Reliability—accurate dispensing over a 50nL to 50µL range, giving high quality assay data Increased flexibility –select rows, columns, or individual wells with the intuitive FILLit Software Easy-to-use—visual, icon-based onboard user interface makes all functions easy to set up, use, and maintain Reduced reagent costs—reliable low volume dispensing, a minimal dead volume and backflush of expensive reagents Application areas: Primary and secondary screening Drug Discovery Assay development Compound management PCR reaction set-up Sequencing

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Optimization of a Bulk Reagent Liquid Handler for Low Volume Dispensing