Automated Sample Preparation of 25-OH-Vitamin D3/D2 from Serum with the AC Extraction Plate and the Tecan Freedom EVO® for the quantification by LC-MS/MS

The Tecan AC Extraction Plate* with TICE™ (Tecan Immobilized Coating Extraction) technology revolutionizes your sample preparation routine. The AC Extraction Plate is a 96-well, deep-well microplate, with each well having a proprietary TICE™ coating. TICE is a material with distinct absorptive properties, and this coating efficiently extracts specific small molecule analytes from aqueous solutions, releasing them when in contact with a suitable organic solvent. A simple pipette and shake sequence, with no filtration, centrifugation or solvent evaporation, is all that is required. The AC Extraction Plate is easily integrated into automated processes, making it a perfect match with Tecan’s Freedom EVO® liquid handling platform. AC Extraction Plate™ Features: Cost-effective – Simple pipette and shake workflow, without labor intensive centrifugation, filtration and solvent evaporation steps. Convenient and fast – Just unpack and start, no conditioning or other preparations needed. Highly reproducible – Pipette and shake routines are easily standardized and automated on a liquid handling platform, eliminating random errors and increasing consistency of results. * For research use only – not for clinical diagnostic applications

Tecan’s Te-Shake orbital mixer offers software-controlled shaking and heating options for microplates, deep-well plates and microfuge tubes. Te-Shake is fully compatible with Tecan’s pipetting platforms, providing automated transport of plates to and from the shaker platform. With multiple configurations available, Te-Shake is sure to fit your laboratory’s specific application needs. Te-Shake is easily integrated into Freedom EVO® or Genesis-based workstations. No external power supply is necessary. Straightforward operation of the device is provided by Tecan’s Pipetting Software Package. On-deck mounting optionally allows pipetting during or between shaking periods. Due to flexible shaking speed and rotation mode, optimal mixing results will always be achieved even for delicate resuspension tasks. Te-Shake highlights: Te-Shake can accommodate virtually any type of microplate or deep-well block The module is available in various configurations that address, for example, heated mixing and/or multiple parallel microplate shaking With its dynamic shaking range, configurable orbit/amplitude and variable rotating direction, Te-Shake allows easy optimization of extended mixing Automated exchange of plates on Te-Shake allows multiple batch processes without user intervention Process scale-up can be realized by parallel use of multiple Te-Shake options on one single workstation Te-Shake applications: Cell lysis processes Bead-based extraction methods Cell metabolism assays Solubilization and homogenization tasks