Comparative Extraction and LC-MS-MS of 10 ng/mL Clenbuterol (R(-) and S(+) enantiomers) in Rat Plasma
1 May 2012

In this application example, rat plasma samples were spiked with clenbuterol (R(-) and S(+) enantiomers) at the level of 10 ng/mL and extracted using three different procedures: Hybrid- SPE-PPT, Protein PPT, and a 9-step SPE procedure optimized for trace level clenbuterol analysis.

Comparisons of sample preparation methods were made in terms of the amount of phospholipids in the sample extract and the overall effect on signal response of clenbuterol enantiomers.

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