RAFT Culture Viability Determination Using the LIVE/DEAD Viability/Cytotoxicity Assay

17 Jan 2013

In this application note the LIVE/DEAD Viability/Cytotoxicity kit from Life Technologies is shown to be an easy and rapid assay that can be used to assess cell viability in a RAFT 3D cell culture. Controls need to be included in the test to ensure that possible background noise is taken into account.

RAFT (Real Architecture for 3D Tissue)

TAP Biosystems

RAFT is a new 3D cell culture system that enables scientists to create complex 3D tissue models in a simple, consistent and reproducible format. RAFT uses physiologically relevant collagen concentrations to create the most natural environment for cells. RAFT enables scientists to create 3D cell cultures in 3 simple steps in less than 1 hour. RAFT can be used to support assay development and secondary screening applications. It can also be used in a broad range of cell biology applications including: stem cell research, oncology research, toxicology and tissue engineering / scaffolds.

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RAFT Culture Viability Determination Using the LIVE/DEAD Viability/Cytotoxicity Assay