ResourceSeparations

Quantification of Exenatide Using Xevo TQD

10 Dec 2012

Exenatide is a large therapeutic peptide that is a synthetic version of Exendin-4, a hormone found in saliva of the Gila monster. The ability to accurately quantify therapeutic peptides in biological fluids requires a selective isolation process, a high-resolution chromatography system and a high-sensitivity detector. This application note demonstrates the capabilities of the Waters Xevo TQD Mass Spectrometer, ACQUITY UPLC H-Class System, sample preparation methods, and column chemistries to achieve a sensitive LC/MS/MS method for the detection and quantification of exenatide in plasma.

Xevo TQ MS

Waters

Waters Xevo™ TQ MS provides the ultimate in quantitative capabilities for your most demanding applications and your most complex sample matrices. It is a highly advanced tandem quadrupole mass spectrometer designed to enable uncomplicated access to superior HPLC and UPLC®/MS/MS performance, delivering the highest levels of sensitivity, selectivity, robustness, speed, and accuracy. Waters Xevo TQ MS is equipped with unique collision cell technology (ScanWave™) to provide new enhanced spectral LC/MS/MS data acquisition capabilities. Unlike conventional tandem quadrupole mass spectrometers, the Xevo TQ MS is designed not only to provide the very best quantitative data, but also to provide you with superior spectral MS/MS information. Xevo TQ MS offers you much more versatility in a single instrument platform that is adaptable to a variety of different UPLC/MS/MS applications both quantitative and qualitative: bioanalysis, ADME screening, food safety, environmental monitoring, forensic, and more. Features: Automated system set up and system checks for simple access Automated instrument optimization and quantitative method development tools for ease-of-use The latest innovations in high efficiency, robust, easy-to-use ZSpray™ atmospheric pressure ion source technology Versatile range of ionization options, including multi-mode ionization capability in a single run Unique, innovative T-Wave™ and ScanWave enabled collision cell technology Footprint that sits easily on your laboratory benchtop Dedicated application managers for your specialized analytical requirements

(14)

Links

Tags

Quantification of Exenatide Using Xevo TQD