Application Note: QuantiFast® Pathogen IC for Evaluation of PCR Inhibition
6 November 2013
Real-time PCR provides a reliable method for detecting viral RNA and DNA or bacterial DNA. But the verification of negative results requires controlling for any PCR malfunctions that may cause false negative results, such as inhibition. The most efficient way to monitor inhibition is the use of an Internal Control (IC). In this article the methodology is described for the combined control of nucleic acid extraction and amplification. In addition, how the IC can be used to evaluate the inhibitory potential of PCR samples.