Application Note: Automated Purification of DNA from Urine for Sensitive PCR of Chlamydia Trachomatis Amplicons
27 November 2013

Development of sensitive and reproducible assays for clinical and life science research of C. trachomatis is essential to monitor and help prevent further spread of this pathogen. DNA was purified from 130 urine research samples using a fully automated protocol on the BioRobot® M48 workstation together with the MagAttract® DNA Mini M48 Kit. Purified DNA performed well in a real-time PCR assay being developed for the amplification of Chlamydia trachomatis DNA. Comparison of this easy-to-use procedure with a commercial C. trachomatis assay and manual DNA purification showed identical sensitivity.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me