Application Note: NanoPro Assay: c-Myc Epitope Tag on EGFP
23 May 2011

Epitope tags are widely used for affinity purification as well as for highly sensitive detection of recombinant proteins. The c-Myc-tag consists of a short peptide sequence (MEEQKLISEEDLLM). In this application note from Cell Biosciences, EGFP was expressed with a c-Myc-tag at either the C- or N-terminus of the protein. Due to the amino acid composition of this tag, a slight acidic shift in the pI of the tagged protein is observed.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions


Already a member?

Log in for full access to the article




Remember me

Tags