Development of a New Real-Time PCR Quantitation System for Human and Y DNA Analysis and Normalization Software

5 Nov 2013

This application note discusses the development of a new technology for real-time quantitative PCR. The technology offers an advantage over currently available systems by simultaneously quantitating both the total human DNA and male-specific DNA within a sample, in addition to an internal PCR control. Simultaneous quantification of autosomal and Y-chromosome DNA means: less variability, less time and more valuable data.

Plexor® HY System

Promega Corp.

  If there is DNA to detect, the Plexor® HY System will show it. Total human DNA. Total male DNA. The power to see it all in a single assay.   The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors and a melt curve function to confirm that the correct product was amplified. Plexor® HY is a sensitive multiplex kit that routinely detects approximately 6.4pg of total DNA. PCR setup is performed at room temperature and is compatible with automated platforms. Plexor® HY DNA Quantitation System Features: Simultaneous quantification of autosomal and Y-chromosomal DNA - Less variability, less time, more valuable data. Consistent and reproducible detection of 6.4pg of DNA - If you can't detect it with Plexor® HY, you can't detect it with your STR system. Internal PCR control and melt-curve analysis - Guard against false negative and false positive results, allowing you to be confident in your data.

(0)

Links

Tags

Development of a New Real-Time PCR Quantitation System for Human and Y DNA Analysis and Normalization Software