Application Note: The Phynex PhyTip Column Method for Obtaining High Concentrations of Active Proteins
13 February 2013

A common problem in the purification of proteins expressed at low concentration is a resulting pure protein that is too dilute for subsequent assays. This application note demonstrates how the PhyNexus two-step purification method, which utilizes PhyTip columns, is suitable for obtaining a desirable yield and purity. The efficiency of elution from a PhyTip column, combined with retention of protein activity, provide a good solution for an automatable concentration process.