Application Note: PhyNexus Columns for Biotinylated Protein Assays
17 June 2013
The challenge of modern target discovery requires the efficient automation of complex assays. The immunoprecipitation (IP) assay has proven quite useful in research discovery to identify components of molecular complexes, discovering protein-protein and protein-nucleic acid interactions, and quantifying an immunogenic response. Protein A PhyTip® columns have been successfully used for this purpose but IP can only be successful if an antibody is available to the peptide or protein of interest In this application note read how PhyNexus Affinity Purification, Gel Filtration columns are the useful tools for small-volume, automated, high throughput purification, separation, and immobilization of biotinylated proteins.