Application Note: Frequency Domain FLIM from Nano to Milliseconds
18 March 2011

Frequency domain FLIM is a method to acquire wide-field FLIM images. Fluorescence lifetimes fall typically within the range of 1 - 10 ns. For much longer lifetimes like 10 μs the optimal frequency is approximately 15 kHz. For these long lifetimes a much lower frequency of the excitation is needed, else the modulation of the emitted signal from the sample is too low for accurate measurements. For coverage of the low frequency range Lambert Instruments developed the LIFA-X.