Screening HDAC Inhibitors – A Workflow Comprising High Throughput and High Content Screening
9 Aug 2013

To allow efficient screening of large compound libraries, intelligent screening workflows are needed that combine different assay formats and multiplex various readouts, including phenotypic information, to provide a more holistic view of compound effects. This application note demonstrates the potential power of combinatorial screening campaigns. An AlphaLISA assay was run on the EnVision Multilabel Plate Reader as a primary screen and a number of active compounds were then selected to be further analyzed and verified in a cellular assay using the Operetta High Content Imaging System.

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