Application Note: Imaging Microtissue Cores using the Opera High Content Screening System
30 Aug 2013
Visualization of 3D microtissues using conventional confocal microscopy is challenging, because tissue opacity, light absorption and scattering prevent imaging at higher planes deep into the core of microtissues. This application note describes a method for detailed analysis of all cell layers within cancer microtissues using the Opera® High Content Screening System with an optical clearance solution. Pre-treatment of microtissues with the aqueous reagent Scale renders tissues optically more transparent, while fully preserving fluorescent signals in the clarified structures.