Application Note: Optimizing the Purification of a Standard Chiral Compound Utilizing a Benchtop, Multi-Purpose, Semi-Preparative to Preparative HPLC System
11 October 2011

The purpose of this study by Gilson, inc. was to optimize the chiral separation of a standard compound using a stand alone, benchtop, preparative chromatography system. Specifically one that is capable of performing separation by either normal phase or reverse phase chromatography with flow rates up to 100 mL/min. Percent recovery, loading capacity, fraction collection parameters, precision, and its percent impurity are reported for trans-Stilbene oxide using normal phase chromatography.

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