Application Note: Optimizing the hERG Assay on the PatchXpress 7000A System for Compounds that Demonstrate Non-specific Binding
6 July 2011

In this application poster eight compounds, astemizole, pimozide, dofetilide, cisapride, terfenadine, flunarizine, quinidine and imipramine were used to evaluate the effect of different assay conditions to overcome the effects of non-specific binding in a hERG assay on the PatchXpress 7000A system. To achieve the best IC50 values, care had to be taken in all phases of compound preparation. Compound binding to plastic container was minimized. Buffers used for the assay also had to be optimized.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me