Application Note: Optical Design of CFX96™ Real-Time PCR Detection System
1 November 2013

In contrast to the optical design of most real-time PCR systems, the CFX96 and CFX384™ real-time PCR detection systems utilize solid-state optical technology with long-lasting light-emitting diodes for fluorescence excitation and photodiodes for detection. Therefore eliminating the need to sacrifice data collection in one of the channels to normalize to a passive reference. In this study, evidence is presented to support the methodology and show the impact of reaction volume of fluorescence chemistry on replicate samples.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me