Application Note: Monitoring Growth of Beer Brewing Strains of Saccharomyces Cerevisiae
3 Jun 2011
In this application note, Biotek describe the use of the Synergy™ H1 Hybrid Multi-Mode Microplate Reader to provide temperature control, solution agitation, and monitor cellular yeast growth using light scatter in 96-well microplates. An overnight culture of strain 2000 Budvar lager yeast was diluted using YPD media. Aliquots of each dilution were pipetted in replicates of 8 into a microplate and their absorbance at 600 nm was determined. Salt, temperature and pH sensitivity, ethanol tolerance and the effects of cell number were analysed.