Application Note: Sequence-Specific DNA Assay
9 January 2013

Measurement of specific DNA sequences can be achieved using the Threshold® System from Molecular Devices. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve. The sequence-specific assay has been used to quantitate PCR products and should be useful for a variety of other applications such as plasmid copynumber determination.