Application Note: IR-based Protein Quantitation Comparison to Colorimetric Assay Quantitation
5 March 2012

This note compares infrared (IR)-based protein quantitation using the Direct Detect™ quantitation system to Bradford and bicinchoninic acid (BCA) colorimetric assays and shows that IR-based quantitation provides results that match concentrations determined by amino acid analysis, even in the presence of detergent or reducing agent.