Application Note: Simple and Effective Generation of Cell-based Assays for Ion Channels, Transporters, and Kinase Screening in Biologically Relevant Cells Using Scalable Transient Transfection
15 August 2014
This poster demonstrates that MaxCyte electroporation provides an extremely effective and flexible means of co-transfecting multiple plasmids to construct a variety of receptors and other assay targets, including GPCRs, ion channels, transporters, and kinases. The MaxCyte STX® Scalable Transfection System uses proprietary flow electroporation to transiently transfect from 5E5 cells in seconds up to 2E10 cells in less than 30 minutes, yielding high levels of transfection efficiency and cell viability. The technology is applicable to a wide range of cell types, including primary cells and stem cells. This poster presents MaxCyte transient-transfected assays including a CFTR transporter assay, a HCS casein kinase assay, and ion channel assays. The results demonstrate that MaxCyte transfection provides a rapid and robust means for conducting cell-based assay screening.