High Speed Separation of Steroid Drug Cortisone Acetate Utilizing Extreme High Pressure Liquid Chromatography (X-LC ® )
1 May 2012

The U.S. Pharmacopeia (USP) method requires that HPLC analysis of components of a cortisone acetate drug should have a resolution greater than 2.2 and the reproducibility of the peak ratio to be not greater than 2.0%. This application note shows the separation of a standard mixture of propyl paraben, butyl paraben and cortisone acetate. The X-LC system provides an analysis time 4 times shorter than conventional HPLC while the resolution was 12.2.

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