IR-based Protein Quantitation Surpasses Colorimetric Assay Quantitation and Results are Independent of Detergents, Reducing Agents and Analysis Time
26 March 2012
Read this application note to learn about a comparison for infrared (IR)-based protein quantitation using the Direct Detect™ quantitation system to Bradford and bicinchoninic acid (BCA) colorimetric assays. Results show that IR-based quantitation provides results that match concentrations, determined by amino acid analysis, even in the presence of detergent or reducing agent. Also it is shown that, unlike colorimetric assays, the calculated protein concentration obtained from IR-based analysis is
unchanged regardless of the time delay between assay and data acquisition.