Application Note: Infrared Detection Decreases Nitrocellulose Autofluorescence and Improves RPPA Assays Signal‐to‐Noise Ratio Over Visible Wavelength Detection
16 August 2013

Reverse Phase Protein Arrays (RPPAs) are a specific type of protein arrays consisting of printed cell lysates useful for cell signaling and biomarker analysis. RPPAs are mainly built on nitrocellulose film slides as they have high binding capacity and viability for native proteins. Until now, RPPA assays with fluorescence detection were limited in sensitivity by high intrinsic background fluorescence normally produced by the nitrocellulose; in this application note learn how it is now possible to increase the Signal to Noise Ratio using Near Infrared (NIR) detection with the InnoScan 710‐IR scanner.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions


Already a member?

Log in for full access to the article




Remember me

Company