Application Note: Improved Sensitivity of Hydrolyzed Urine Samples Using β-Gone™ β-Glucuronidase Removal Products
12 February 2017

When the drugs exit the body through urine they are still in their glucuronide form and before chromatographic analysis the glucuronide bond must hydrolysed by β-glucuronidase, however residual β-glucuronidase and drug compounds can build up and negatively impact on the selectivity of the column. This application note describes a method for removing residual enzyme using β-Gone β-Glucuronidase Removal Products. This work demonstrates how these products can improve sensitivity while adding little variability to the results.