Improved Sensitivity of Hydrolyzed Urine Samples Using β-Gone™ β-Glucuronidase Removal Products

When the drugs exit the body through urine they are still in their glucuronide form and before chromatographic analysis the glucuronide bond must hydrolysed by β-glucuronidase, however residual β-glucuronidase and drug compounds can build up and negatively impact on the selectivity of the column. This application note describes a method for removing residual enzyme using β-Gone β-Glucuronidase Removal Products. This work demonstrates how these products can improve sensitivity while adding little variability to the results.