DNA Barcoding from Fish Tissues
6 Aug 2014
Routine DNA extraction from fish tissues and subsequent PCR for DNA barcoding is time consuming and laborious, limiting the potential for rapid, high-throughput analysis. This application note describes a fast, convenient and efficient alternative for the routine extraction and amplification of DNA from fish fins and flesh to enable species identification. The KAPA Express Extract system facilitates optimal tissue lysis and preservation of DNA extracts. The method eliminates excessive handling, thereby generating PCR-ready DNA in as little as 15 min. KAPA2G Robust HotStart ReadyMix contains an engineered DNA polymerase tolerant to common PCR inhibitors, offering reliable amplification of DNA extracted from fish tissues. With the combination of KAPA Express Extract and KAPA2G Robust HotStart ReadyMix, reliable DNA amplification from fish tissues can be achieved in ≤2 hours.