Corning® Synthemax™ Surface: A Tool for Feeder-Free, Xeno-Free Culture of hESCs
22 Jun 2011

In this application note from Corning, a tool for hESC culture is assessed as an alternative to feeder cells or complex mixtures of proteins extracted from mouse tumours. A novel synthetic surface that permits consistent long-term self-renewal of multiple hESC lines in defined, xeno-free media and differentiation of cells to functional cardiomyocytes is presented. Long-term, multi-passage self-renewal, maintenance of a pluripotent state, and differentiation capabilities are analysed.

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