Application Note: iCell® Cardiomyocytes – Immunofluorescent Labeling Application Protocol
17 July 2013

Immunofluorescent labeling is a straight-forward technique for assessing the presence and the subcellular localization of antigens and/or proteins. Several labeling methods are available depending on the biological sample, cell preparation, and availability of antibodies against the target. The protocol presented here has demonstrated utility in labeling cardiac troponin T, myosin light chain, sarcomeric alpha-actinin, connexin-43, and N-cadherin in iCell® Cardiomyocytes. This protocol should serve as a guide for immunofluorescent labeling other cardiac proteins.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me