Analyzing the Role of Different Adhesion Molecules and Chemokines Involved in Inflammation
24 Sept 2012This application note analyzes the role of different adhesion molecules and chemokines involved in various stages of inflammation under physiological flow conditions. Using Cellix’s biochips and Mirus pumping system, THP-1, monocyte and PBMC adhesion to VCAM-1; THP-1, monocyte and PBMC rolling on E-selectin; and respective adhesion blockades is investigated. THP-1 adhesion to HUVECs, correlating adhesion assay results with adhesion molecule expression levels on HUVECs from flow cytometry data, is also investigated. This method also investigates THP1 and PBMC adhesion to TNFα stimulated HUVECs and the effect of blocking antibodies (anti-VCAM-1; anti E-selectin; anti ICAM-1 and a combination of all three).
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Vena8 Fluoro+ Biochip: microfluidic flow chamber
Cellix LtdThe Vena8 Fluoro+™ biochip is ideal for studying cell receptor-ligand interaction and is compatible with confocal microscopy. As cells flow through the microcapillary of the Vena8 Fluoro+™ biochip, the cell surface receptors may interact with adhesion molecules or ligands which coat the microcapillary walls. Ideal for use with fluorescence immunostaining, confocal microscopy and for applications such as thrombosis.