Application Note: Cell Sorting Based on RNA detection in Living Cells Using SmartFlare™ RNA Detection Reagents
15 April 2013
Identifying cell types and sorting cells based on RNA expression levels without any transfection reagents or intrusive sample preparation can drastically improve live cell sorting efficiency, physiological relevance, and post-sorting survival rate. In this application note it is demonstrated that SmartFlare™ RNA detection probes, which are capable of detecting levels of RNA inside living cells, can be used to sort and further propagate live cell populations purely based on gene expression levels or in combination with surface markers detected with antibodies.