2500 Protein Identified from a Human Cell Lysate with Complementary MALDI and ESI Data
11 Oct 2011

This application note shows that recent improvements in both MS instrumentation as well as nanoLC separation allows 1500-2000 protein IDs to be made on each of Bruker´s ESI and MALDI mass spectrometry platforms. Moreover, Bruker’s new PRIME concept enabled seamless combination of ESI and MALDI data for further increased sequence coverage as well as enhanced protein identification rate. Merged ESI and MALDI data led to the identification of nearly 2500 proteins.

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