Application Note: Automation of HTRF® elF4E Kinase Assay Using a 3D Tumoroid-Based Cell Model
11 September 2013

This application note demonstrates an in vitro microplate assay that can quantify total, as well as phosphorylated eIF4E. The assay was performed wherein cells were cultured using a novel 3D culture system, RAFT™. All dispensing and removal steps were performed by the MultiFlo™ FX Microplate Dispenser.