Application Note: Simultaneous Extraction of Ethyl Glucuronide and Ethyl Sulfate from Urine with EVOLUTE AX Prior to LC-MS-MS Analysis
15 August 2012

This application note describes the simultaneous extraction of the alcohol metabolites ethyl glucuronide (EtG) and ethyl sulfate (EtS) from human urine using Biotage EVOLUTE AX columns in a range of column sizes followed by LC-MS-MS analysis. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS.