Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System

High purity protein is a common requirement for biochemical and structural studies. A common approach is to recombinantly express an affinity-tagged version of the protein of interest. This is, however, not always a viable option. This application note describes protein purification workflow development for untagged proteins and introduces a new indicator of method performance, the purity quotient difference (PQD).