Application Note: Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP Laboratory Automation Workstation
24 April 2013

The well-documented ability of stem cells to differentiate into various cell lineages generates tremendous potential for cell-based treatments. Flow cytometry is a common method for detection of various cell types, but requires automated sample preparation for use in higher throughput situations. This application note demonstrates how the Biomek NXP Span-8 Automation Workstation can be used for flow cytometry sample preparation to determine the efficiency of cardiomyocyte differentiation from murine embryonic stem cells. The workflow includes fixation, permeabilization, blocking, and antibody staining in a 96-well plate format.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me