Automated Analysis of Proteins Using the LabChip GX System
7 Jul 2011
In this application note from Caliper Life Sciences an assay and microfluidics platform are used for protein analysis as an alternative to SDS-PAGE. Carbonic anhydrase samples were prepared at concentrations between 2000 ng/μL and 2.5 ng/μL and the resulting electropherograms were superimposed to illustrate separation sensitivity and reproducibility. E.coli cells were used to over express a ~40 kDa protein. E. coli proteins were analysed at various stages of the protein expression process in whole cell lysate, soluble and insoluble lysate fractions and Ni bead purified samples.