Application Note: A High-Throughput, Homogeneous, FRET-Based Assay to Detect Bacterial Membrane-Bound Enzyme
3 December 2013

The activity of an integral membrane protein (MraY) was measured using a FRET-based assay. The FRET donor is coupled to the translocase substrate while the acceptor is embedded in micelles that also contain the enzyme. The assay is useful for searching inhibitors that represent drug targets. The ratiometric measurement was performed simultaneously in 384-well format using the PHERAstar HTS microplate reader from BMG LABTECH.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me