ResourceLife Sciences
Multi-color Immunofluorescence in Brain Tissue Using Primary Antibodies Raised in the Same Host Species
22 Nov 2011A novel technique for triple-label immunofluorescence IHC is described in this application note. The technique includes modification of one primary antibody with biotin and a second primary antibody with DIG. This technique from R and D systems provides good spectral separation of colors depicting different antigens of interest, and provides significant convenience to researchers who have at their disposal only primary antibodies raised in the same host species.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Immunofluorescence