Extraction of DNA from Cigarette Butts using Proteinase forensicGEM™
11 Feb 2013Cigarette butts are a common trace sample at crime scenes and obtaining DNA profiles from this evidence is an important capability in the forensic science repertoire. In this application note it is shown that the forensicGEM™ Cigarette method is a quick and easy method for processing cigarette butts. While being substantially faster than current organic extraction methods, it is also capable of producing full profiles using AmpFlSTR® Identifiler® – a multiplex fingerprinting system that is particularly sensitive to inhibition.
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forensicGEM™
ZyGEM Corporation LimitedforensicGEM™ has been formulated for high-throughput DNA extraction from crime samples. The forensicGEM™ kits have been specifically designed to be compatible with high-multiplex STR profiling kits and all reagents are rigorously Quality Controlled to test for efficacy and for the absence of human DNA. ZyGEM technology is ideally suited to the large-scale batch processing of reference samples for forensic analysis. Validated by ESR New Zealand, one of the largest forensics laboratories in the world, forensicGEM™ is a rapid, high throughput processing technology for storage card and buccal swab reference samples. ZyGEM also offers a comprehensive range of optimized kits for the preparation of crime scene and CODIS samples. All forensicGEM™ kits are rigorously tested at a leading forensic laboratory for efficacy and purity. The basis of all of the kits is the forensicGEM™ proteinase, but special formulations have been developed for different substrates forensicGEM™ DNA EXTRACTION HAS MANY ADVANTAGES: Closed–tube (protects sample integrity) Hands–off (saves labor costs) One-step (maximizes yields and so increases the chance of obtaining a usable profile) Easily automated (compatible with most low cost, off-the-shelf liquid handling robots) Validated for forensic analysis