Application Note: Evaluation of X‐MAN™ B‐Raf (V600E) Standard in COLD‐PCR Assay for detection of
12 April 2012

Serine threonine kinase v-RAF murine sarcoma viral oncogene homolog B1 (BRAF) mutations are frequent in benign and malignant human tumors, and are emerging as an important biomarker. This application note describes a comparison between a conventional high-resolution melt detection method and a coamplification-at-lower-denaturation-temperature PCR (COLDPCR) method.