Elevations of Intracellular Kinases AKT1, GSK3β, JNK2 and ERK2 in Primary Breast Cancer Tissue Quantified with a Novel Multi-panel of Single Molecule Immunoassays

This poster presents the testing of adjacent normal and primary breast cancer tissue lysates with a novel panel of eight immunoassays for quantifying the total and phosphorylated forms of AKT1, GSK3β, JNK2 and ERK2. Analytical sensitivity, inter-assay precision, linearity and specificity were determined and compared to corresponding ELISA assays.