Determination of Endogenous Cellular Kinase Activity Through the Use of an Automated, High Throughput AlphaScreen-Based Assay
1 Oct 2012

In this application note from BioTek, the use of the AlphaScreen® SureFire® assay for the assessment of kinase signalling pathway modulation is described. The assay procedure was easily automated in low-volume 384-well format using the aspiration and non-contact dispensing capacilities of the EL406™ or MultiFlo™. The laser-based excitation and filter-based detection system of the Synergy™ NEO microplate reader was used to easily and efficiently quantify the emitted signal from the AlphaScreen® acceptor beads.

Popular tags in SelectScience

Buying guides

Write a Review

Join the Global SelectScience Community Today! It’s FREE!

Helping you make informed decisions about the latest lab products and technologies:

  • Be the first to learn about new technologies
  • 10,000+ trusted product reviews from your peers
  • Compare 500,000 products and manufacturers
  • Get exclusive buying tips from experts
  • Find solutions fast: 10,000+ Application Notes, Webinars and Videos

Connect with 250,000 scientists... All in one place!

Become a member today!