Application Note: Advances in Size Exclusion Chromatography for the Analysis of Macromolecular Proteins
2 April 2013

Size exclusion chromatography has been the preferred method for the analysis of proteins based on size. This application note demonstrates how by combining 450 Å sub 3-µm packing materials with a low dispersion ACQUITY UPLC H-Class System, separations with improved resolution and high-throughput of SE-UPLC can be realized for macromolecular proteins and highly aggregated proteins with molecular weights of up to approximately 2000 kDa.