Advances in Size Exclusion Chromatography for the Analysis of Macromolecular Proteins
1 Apr 2013Size exclusion chromatography has been the preferred method for the analysis of proteins based on size. This application note demonstrates how by combining 450 Å sub 3-µm packing materials with a low dispersion ACQUITY UPLC H-Class System, separations with improved resolution and high-throughput of SE-UPLC can be realized for macromolecular proteins and highly aggregated proteins with molecular weights of up to approximately 2000 kDa.
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BEH (Ethylene Bridged Hybrid) Particles for ACQUITY UPLC
WatersThe 1.7 µm Ethylene Bridged Hybrid [BEH] particle is one of the key enablers behind UPLC® technology.It is available in three different pore sizes [130Å, 200Å and 300Å] and several bonded phases for reversed-phase and hydrophilic interaction chromatography and is applicable from small molecule to large biopharmaceutical analysis. Due to the intrinsic chemical stability of hybrid particle technology, a wider usable pH range [pH 1-12] can be employed, enabling a versatile and robust separation technology for method development. BEH particle technology is available in HPLC particle sizes [2.5, 3.5, 5 and 10 µm] in the XBridge® family of HPLC columns, enabling seamless transfer between HPLC and UPLC technology platforms.